The Brownian motion of the HMM molecules may release more head domains from the glass surface at higher temperature without releasing the attached end.
The increased thermal motion of the thin filament could reach a larger number of HMM molecules, thereby forming the increased number of cross-bridges at higher temperature.
The interaction between actin and myosin may be promoted at higher temperatures, because it is an endothermic reaction.
However, whether this increase in the number of available cross-bridges is a physiological phenomenon is still an open question.
Kawai, M., et al., Temperature change does not affect force between regulated actin filaments and heavy meromyosin in single-molecule experiments. J Physiol, 2006. 574(Pt 3): p. 877-87.
